EGLN1/PHD2 Antibody

• Supplier: Novus Biologicals
• Catalog: NB100-137
• Prices: $99.00, $329.00
• Sizes: 25 µg, 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB Simple Western FC ICC/IF IHC IHC-P IP
• Species Reactivities: Human, Rat, Rabbit
• Antigen: The epitope recognized by this antibody maps to a region between residues 1 and 50 of human PHD2/HIF Prolyl Hydroxylase 2 using the numbering given in entry NP_071334.1 (GeneID 54583).
• Purity/Format: Immunogen affinity purified
• Blocking Peptide: EGLN1/PHD2 Peptide
• Description: Rabbit Polyclonal
• Gene: EGLN1
• Conjugate: Unconjugated

Product images

Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Detection of Human PHD2 by Western Blot. Samples : Recombinant epitope-tagged PHD1, PHD2 or PHD3 (10 ng/lane) or whole cell lysate from HeLa cells. Antibody : Affinity purified rabbit anti-PHD2 used at 1 ug/ml. Detection : Chemiluminescence.

Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody [NB100-137] - Staining of endogenous PHD2 in U2OS cells. Image from verified customer review.

Immunohistochemistry: EGLN1/PHD2 Antibody [NB100-137] - Staining of Vascular Endothelium 40X in Lung.

Flow Cytometry: EGLN1/PHD2 Antibody [NB100-137] - Detection of PHD2. 1 million Jurkat cells were fixed, permeabilized, and stained with 3.0 ug/ml anti-PHD2 NB100-137 in a 150 ul reaction. Isotype control (black), anti-MLL1 (red).

Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Analysis of PHD2 expression in human prostate cancer cell lines. Image from verified customer review.

Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Analysis of PHD2 in Hep3B cell lysate.

Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Detection of PHD2 human glioblastoma cells. Image from verified customer review.

Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Analysis of PHD2 in normoxia (lane 1) or hypoxia (lane 2) treated SK-N-BE(2) cells using anti-PHD2 antibody. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody [NB100-137] - Staining of PHD2 in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Simple Western: EGLN1/PHD2 Antibody [NB100-137] - Simple Western lane view shows a specific band for PHD2/HIF Prolyl Hydroxylase 2 in 0.5 mg/ml of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.