Western Blot: LRRK2 Antibody [NB300-268] - Detection of LRRK2 in 50 ug of crude bovine brain membrane.
Immunocytochemistry/Immunofluorescence: LRRK2 Antibody [NB300-268] - Mouse CAD cells transfected with Human wild-type LRRK-2 (1:2,000).
Immunohistochemistry: LRRK2 Antibody [NB300-268] - Brain, Putamen, Neurons and Glia 60x.
Flow Cytometry: LRRK2 Antibody [NB300-268] - Analysis using the Alexa Fluor (R) 647 conjugate of NB300-268. Staining of LRRK2 in normal human peripheral blood cells (anticoagulated Lithium-Heparin) using anti-LRRK2 antibody conjugated with AF647. The primary antibody was used at a dilution of 1:200 and incubated for 20 min at room temperature. Image from verified customer.
Western Blot: LRRK2 Antibody [NB300-268] - Detection of LRRK2 in HeLa whole cell lysate (RIPA) using NB300-268. 1:5000 dilution, 1 minute ECL detection.
Immunocytochemistry/Immunofluorescence: LRRK2 Antibody [NB300-268] - LRRK2 antibody was tested in SH-SY-5Y cells at a 1:200 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red), and DAPI (Blue), respectively.
Immunohistochemistry-Frozen: LRRK2 Antibody [NB300-268] - Staining as described in PMID 24312256. Image from confirmed customer review.
Immunoprecipitation: LRRK2 Antibody [NB300-268] - Immunoprecipitation of LRRK2 followed by an autophosphorylation assay using NB300-268. Lane 1 Rabbit IgG, Lanes 2 and 3 NB300-268.