Western Blot: Nanog Antibody [NB100-58842] - Whole cell lysate (5, 15, and 50 ug) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunocytochemistry/Immunofluorescence: Nanog Antibody [NB100-58842] - Nanog antibody was tested in DGCR8 knockout Mouse embryonic stem cells (NBA1-19349) with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry-Paraffin: Nanog Antibody [NB100-58842] - FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-Nanog (NB100-58842) used at adilution of 1:250. Detection: DAB staining.
Western Blot: Nanog Antibody [NB100-58842] - Mouse Nanog.
Immunohistochemistry-Paraffin: Nanog Antibody [NB100-58842] - FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-Nanog used at a dilution of 1:500 (2ug/ml). Detection: DAB