Ferroportin/SLC40A1 Antibody

• Supplier: Novus Biologicals
• Catalog: NBP1-21502
• Prices: $149.00, $349.00
• Sizes: 25 µl, 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB FC ICC/IF IHC IHC-P
• Species Reactivities: Human
• Antigen: Synthetic peptide made to an internal portion of human Ferroportin 1 (within residues 250-300). [Swiss-Prot# Q9NP59]
• Purity/Format: Immunogen affinity purified
• Blocking Peptide: Ferroportin/SLC40A1 Blocking Peptide
• Description: Rabbit Polyclonal
• Gene: SLC40A1
• Conjugate: Unconjugated

Product images

Western Blot: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Human intestine lysate.

Immunocytochemistry/Immunofluorescence: Ferroportin/SLC40A1 Antibody [NBP1-21502] - HepG2 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Ferroportin 1 at 5.0 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: Ferroportin/SLC40A1 Antibody [NBP1-21502] - IHC-P detection of FPN1 / SLC40A1 protein in murine small intestinal section using Ferroportin 1 antibody at a dilution of 1:200. The antibody primarily developed a membranous staining pattern in the intestinal epithelial cells.

Flow Cytometry: Ferroportin/SLC40A1 Antibody [NBP1-21502] - An intracellular stain was performed on HepG2 cells with Ferroportin/SLC40A1 antibody NBP1-21502AF647 (blue) and a matched isotype control NBP2-24893AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Immunohistochemistry-Paraffin: Ferroportin/SLC40A1 Antibody [NBP1-21502] - IHC-P detection of FPN1 / SLC40A1 protein in murine liver section using Ferroportin 1 antibody at a dilution of 1:200. The representative image shows intense staining in the cellular membranes, whereas, a relatively milder postivity was observed in the cytoplasm of hepatocytes.

Flow Cytometry: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Histogram of Ferroportin staining of human spleen macrophages using Alexa Fluor 700 conjugated Ferroportin antibody (red, catalog# NBP1-21502AF700) and isotype control (grey) as a negative control. Image from verified customer review.

Flow Cytometry: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Analysis using the PE conjugate of NBP1-21502. Staining of Ferroportin in human B lymphocytes in peripheral blood using PE conjugated anti-Ferroportin antibody. Image from verified customer review.