SirT6 (D8D12) Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 12486
• Prices: $246.00
• Sizes: 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D8D12
• Applications: WB IP ICC/IF
• Species Reactivities: Human, Mouse, Rat, Monkey
• Antigen: Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to full-length mouse SirT6 protein.
• Description: Rabbit Monoclonal
• Gene: SIRT6

Product images

Western blot analysis of extracts from various cell lines using SirT6 (D8D12) Rabbit mAb.

Western blot analysis of extracts from HCT 116 cells, expressing either non-targeting shRNA (shNT) or shSirT6, using SirT6 (D8D12) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western blot analysis of extracts from SirT6 wild-type (WT) and knockout (KO) mouse embryonic fibroblasts (MEF) using SirT6 (D8D12) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). SirT6 WT and KO MEF were kindly provided by Dr. David Lombard, University of Michigan.

Immunoprecipitation of SirT6 from HCT 116 cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 3) or SirT6 (D8D12) Rabbit mAb (lane 2). Lane 1 is 10% input. Western blot analysis was performed using SirT6 (D8D12) Rabbit mAb.

Confocal immunofluorescent analysis of HCT 116 cells, expressing either non-targeting shRNA (shNT) (left) or shSirT6 (right), using SirT6 (D8D12) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).