Immunohistochemistry: HUWE1 Antibody [NB100-652] - Sample: FFPE section of human ovarian carcinoma.~~Antibody: Affinity purified rabbit anti-Lasu1/Ureb1~ used at a dilution of 1:1,000 (1ug/ml).~ Detection: DAB Counterstain: IHC Hematoxylin (blue).
Western Blot: HUWE1 Antibody [NB100-652] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP) from 293T cells. Ureb1/Lasu1 was less efficiently immunoprecipitated by another Ureb1/Lasu1 antibody.
Immunohistochemistry-Paraffin: HUWE1 Antibody [NB100-652] - Human ovarian carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).
Proximity Ligation Assay: HUWE1 Antibody [NB100-652] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human ATM (NB100-271) and rabbit anti-human Lasu1 (NB100-652). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.
Proximity Ligation Assay: HUWE1 Antibody [NB100-652] - Secondary-conjugate Duolink In Situ PLA in Hela cells. goat anti-human USP7 (NBP2-22295) and rabbit anti-human Lasu1 (NB100-652). Image merged from DAPI (2ms), FITC/Green (25ms) and Red (200ms) exposures, 40X magnification. Control images may not have counterstained cytoplasm.