Immunocytochemistry/Immunofluorescence: SIAH2 Antibody (24E6H3) [NB110-88113] - Immunofluorescent staining of SIAH in ovarian carcinoma (OVCR3)cells using NB110-88113.
Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113] - Staining of SIAH in human colorectal adenocarcinoma using NB110-88113. Staining of SIAH was not observed in normal colon epithelium. Photos courtesy of Dr. Amy Tang, Mayo Clinic Cancer Center.
Flow (Intracellular): SIAH2 Antibody (24E6H3) [NB110-88113] - Human HepG2 cell line was stained with Mouse Anti- SIAH2 Monoclonal Antibody (Catalog # NB110-88113, filled histogram), or Mouse IgG1 isotype control (Catalog # MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).