Western Blot: VPS35 Antibody [NB100-1397] - (1ug/ml) staining of Human Brain (Cerebellum) (A) and Rat Brain (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry: VPS35 Antibody [NB100-1397] - Staining of PFA-fixed and saponin permeabilized SHSY5Y and detected with FITC in confocal microscopy. Data obtained from Dr. M. Schallburg Nielsen, Aarhus University Denmark.
Immunohistochemistry-Paraffin: VPS35 Antibody [NB100-1397] - staining of paraffin embedded Human Testis showing A) Epithelial cells of the epididymis and B) Some leydig cells. Microwaved antigen retrieval with A)Tris/EDTA buffer pH9 at 3ug/ml or B)Citrate buffer pH6 at 10ug/ml., HRP-staining. Data obtained
Western Blot: VPS35 Antibody [NB100-1397] - Analysis (at 0.05ug/ml) of Human Brain Cerebellum (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunocytochemistry: VPS35 Antibody [NB100-1397] - Staining of PFA-fixed and saponin permeabilized HEK293 and detected with FITC in confocal microscopy. Data obtained from Aarhus University Denmark.