Western blot analysis of extracts from various cell lines using SIX1 (D4A8K) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunoprecipitation of SIX1 protein from A-204 cell extracts using Rabbit DA1E mAb IgG XP ® Isotype Control #3900 (lane 2) or SIX1 (D4A8K) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using SIX1 (D4A8K) Rabbit mAb.
Confocal immunofluorescent analysis of A-204 (positive, left) and Caco-2 (negative, right) cells, using SIX1 (D4A8K) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red).
Flow cytometric analysis of A-204 cells (green), MCF7 cells (red), and LNCaP cells (blue) using SIX1 (D4A8K) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody.