CRM1 Antibody

• Supplier: Novus Biologicals
• Catalog: NB100-79802
• Prices: $359.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB Simple Western ICC/IF IHC IHC-P IP PLA
• Species Reactivities: Human
• Antigen: The immunogen recognized by this antibody maps to a region between residue 1025 and the C-terminus (residue 1071) of human Chromosome Region Maintenance 1 (Exportin 1) (NP_003391.1).
• Purity/Format: Immunogen affinity purified
• Description: Rabbit Polyclonal
• Gene: XPO1
• Conjugate: Unconjugated

Product images

Immunohistochemistry: CRM1 Antibody [NB100-79802] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-CRM1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Western Blot: CRM1 Antibody [NB100-79802] - A) Whole cell lysate from HeLa (5, 15 and 50 mcg), Ramos (50 mcg) and mouse NIH3T3 (50 mcg) cells. B) Whole cell lysate (1 mg/IP; 1/4 of reaction loaded/lane) from HeLa cells. NB100-79802 used at 0.1 mcg/ml for WB (A and B) and at 3 mcg/mg lysate for IP. CRM1 was also less efficiently immunoprecipitated by NB100-79811, which recognize upstream epitopes on CRM1.

Immunohistochemistry: CRM1 Antibody [NB100-79802] - FFPE section of mouse squamous cell carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.

Immunohistochemistry-Paraffin: CRM1 Antibody [NB100-79802] - Human metastatic lymph node. Antibody used at a dilution of 1:5,000 (0.2ug/ml).

Immunohistochemistry: CRM1 Antibody [NB100-79802] - Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-CRM1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB

Simple Western: CRM1 Antibody [NB100-79802] - Simple Western lane view shows a specific band for CRM1 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Proximity Ligation Assay: CRM1 Antibody [NB100-79802] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human ATM (NB100-271) and rabbit anti-human CRM1 (NB100-79802). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.

Proximity Ligation Assay: CRM1 Antibody [NB100-79802] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human CHK1 (NB100-274) and rabbit anti-human CRM1 (NB100-79802). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.

Proximity Ligation Assay: CRM1 Antibody [NB100-79802] - Secondary-conjugate Duolink In Situ PLA in Hela cells. goat anti-human CHK1 (NB100-311) and rabbit anti-human CRM1 (NB100-79802). Image merged from DAPI (2MS), FITC/Green (25ms) and TRITC/Orange (250ms) exposures, 40X magnification. Control images may not have counterstained cytoplasm.

Proximity Ligation Assay: CRM1 Antibody [NB100-79802] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RCC1 (NB110-40590) and rabbit anti-human CRM1 (NB100-79802). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.