Western Blot: MED12 Antibody [NB100-2357] - Detection of Human and Mouse Med12 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Med12 antibody NB100-2357 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: MED12 Antibody [NB100-2357] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MED12 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: MED12 Antibody [NB100-2357] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. NB100-2357 used for WB at 0.1 mcg/ml (A) and at 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).
Immunohistochemistry-Paraffin: MED12 Antibody [NB100-2357] - Human breast carcinoma.
Immunohistochemistry-Paraffin: MED12 Antibody [NB100-2357] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MED12 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: MED12 Antibody [NB100-2357] - FFPE section of human breast adenocarcinoma. Affinity purified rabbit anti-MED12 used at a dilution of 1:250.