Claspin Antibody

• Supplier: Novus Biologicals
• Catalog: NB100-247
• Prices: $359.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ICC/IF IHC IHC-P IP PLA
• Species Reactivities: Human
• Antigen: The immunogen recognized by this antibody maps to a region between residues 450 and 500 of human Claspin using the numbering given in entry AAG24515.1 (GeneID 63967).
• Purity/Format: Immunogen affinity purified
• Description: Rabbit Polyclonal
• Gene: CLSPN
• Conjugate: Unconjugated

Product images

Western Blot: Claspin Antibody [NB100-247] - Detection of Human Claspin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-Claspin antibody NB100-247 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry: Claspin Antibody [NB100-247] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-Claspin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Western Blot: Claspin Antibody [NB100-247] - Detection of human Claspin on HeLa whole cell lysate using NB100-247.

Proximity Ligation Assay: Claspin Antibody [NB100-247] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RAD17 (NB100-272) and rabbit anti-human Claspin (NB100-247). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.

Proximity Ligation Assay: Claspin Antibody [NB100-247] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human CKH1 (NB100-274) and rabbit anti-human Claspin (NB100-247). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.

Proximity Ligation Assay: Claspin Antibody [NB100-247] - Secondary-conjugate Duolink In Situ PLA in MCF-7 cells. goat anti-human USP7 (NBP2-22295) and rabbit anti-human Claspin (NB100-247). Image merged from DAPI (2ms), FITC/Green (25ms) and TRITC/Orange (250ms) exposures, 40X magnification. Control images may not have counterstained cytoplasm.

Proximity Ligation Assay: Claspin Antibody [NB100-247] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human ATM (NB100-271) and rabbit anti-human Claspin (NB100-247). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.

Proximity Ligation Assay: Claspin Antibody [NB100-247] - Secondary-conjugate Duolink In Situ PLA in Hela cells. goat anti-human CHK1 (NB100-311) and rabbit anti-human Claspin (NB100-247). Image merged from DAPI (2ms) and FITC/Green (25ms) and TRITC/Orange (250ms) exposures, 40X magnification. Control images may not have counterstained cytoplasm.

Immunoprecipitation: Claspin Antibody [NB100-247] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Claspin antibody used for IP at 6 ug/mg lysate. For blotting immunoprecipitated Claspin,was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.