CLSPN

Gene Symbol CLSPN
Entrez Gene 63967
Alt Symbol -
Species Human
Gene Type protein-coding
Description claspin
Other Description claspin homolog|hClaspin
Swissprots Q5VYG0 Q8IWI1 Q2KHM3 A6NFL4 Q6P6H5 Q1RMC6 Q9HAW4
Accessions EAX07401 EAX07402 Q9HAW4 AF297866 AAG24515 AK308193 BC018670 AAH18670 BC038991 AAH38991 BC062215 AAH62215 BC113116 AAI13117 BC115023 BC115024 BC115025 AAI15026 BC115026 AAI15027 BC115027 BC137279 AAI37280 BC140789 AAI40790 BG109920 XM_011541938 XP_011540240 XM_011541939 XP_011540241 XM_011541940 XP_011540242 XM_011541941 XP_011540243 XM_011541942 XP_011540244 XM_011541943 XP_011540245 NM_001190481 NP_001177410 NM_022111 NP_071394
Function Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR- dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S- phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks. {ECO:0000269|PubMed:12766152, ECO:0000269|PubMed:15096610, ECO:0000269|PubMed:15190204, ECO:0000269|PubMed:15226314, ECO:0000269|PubMed:15707391}.
Subcellular Location Nucleus {ECO:0000269|PubMed:12766152}.

Claspin Antibody - 2800 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of HEL, SH-SY5Y and NCCIT cells using Claspin Antibody.

Claspin (D-20) - sc-27297 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Claspin (D-20): sc-27297. Western blot analysis of Claspin expression in non-transfected: sc-117752 (A) and mouse Claspin transfected: sc-125137 (B) 293T whole cell lysates.

Claspin (Q-20) - sc-27296 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Claspin (Q-20): sc-27296. Western blot analysis of Claspin expression in HeLa (A), HCT 116 (B), MCF7 (C) and ZR-75-1 (D) whole cell lysates.

Claspin (H-300) - sc-48771 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Claspin (H-300): sc-48771. Western blot analysis of Claspin expression in non-transfected: sc-117752 (A) and mouse Claspin transfected: sc-125137 (B) 293T whole cell lysates.

p-Claspin (Ser 945) - sc-31718 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
p-Claspin (Ser 945): sc-31718. Immunoperoxidase staining of formalin fixed, paraffin-embedded human bone marrow tissue showing nuclear staining of a subset of hematopoietic cells.

Anti-Claspin antibody - ab3720 from Abcam

Prices $401.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Claspin with ab3720 at 1/1000 (1µg/ml). Detection: DAB.

Anti-Claspin antibody - ab3721 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-Claspin antibody - ab83485 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Claspin antibody (ab83485) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear LysateLane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 5 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 7 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Claspin antibody - ab3719 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-Claspin antibody - ab94945 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Claspin antibody (ab94945) at 1/500 dilution + 293T cell lysate

Anti-CLSPN / Claspin Antibody (aa725-775) - LS-C285987 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human Claspin by Immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-Claspin used at a dilution of 1:1000 (1 Detection: DAB.

Anti-CLSPN / Claspin Antibody - LS-C48693 from LifeSpan Bioscience

Prices $410.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CLSPN / Claspin Antibody (aa450-500) - LS-C79378 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-CLSPN / Claspin Antibody (aa900-950) - LS-C79379 from LifeSpan Bioscience

Prices $575.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-CLSPN / Claspin Antibody (aa900-950) - LS-C285988 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human Claspin by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Claspin antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. Claspin was also immunoprecipitated by a previous lot of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Anti-CLSPN / Claspin Antibody (aa450-500) - LS-C286206 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human Claspin by Western Blot. Sample: Whole cell lysate (20 ug) from HeLa cells. Antibody: Affinity Purified Rabbit anti-Claspin used at 1 or 0.5 ug/ml with (+) or without (-) blocking peptide. Detection: Chemiluminescence.

Anti-CLSPN / Claspin Antibody - LS-C137266 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Claspin Antibody - NB100-247 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Claspin Antibody [NB100-247] - Detection of Human Claspin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-Claspin antibody NB100-247 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Claspin Antibody - AF3310 from Novus Biologicals

Prices $329.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot Claspin Antibody

Claspin Antibody (485508) - MAB3310 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 485508
Western blot shows lysates of HCT‑116 human colorectal carcinoma cell line and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Human Claspin Monoclonal Antibody (Catalog # MAB3310) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Claspin at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Claspin Antibody - NB100-246 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Claspin Antibody [NB100-246] - Whole cell lysate (20 ug) from HeLa cells. Antibody used at 1 or 0.5 ug/ml with (+) or without (-) blocking peptide.

Claspin Antibody - NB100-248 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Claspin Antibody [NB100-248] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Affinity purified rabbit anti-Claspin antibody NB100-248 (lot number 2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. Claspin was also immunoprecipitated by a previous lot (lot number 1) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Claspin Antibody [Biotin] - BAF3310 from Novus Biologicals

Prices $390.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

Anti-Claspin antibody produced in rabbit - C7992 from Sigma-Aldrich

Prices $77.90, $435.50
Sizes 25 µl, 200 µl
Host Rabbit
Clonality Polyclonal

Anti-Claspin (C-terminal) antibody produced in rabbit - C7867 from Sigma-Aldrich

Prices $77.90, $443.00
Sizes 25 µl, 200 µl
Host Rabbit
Clonality Polyclonal
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