Endoglin/CD105 was detected in immersion fixed rat mesenchymal stem cells using Goat Anti-Mouse Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1320) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Endoglin/CD105 was detected in immersion fixed frozen sections of mouse embryo (E13-15) using Goat Anti-Mouse Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1320) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Simple Western lane view shows lysates of bEnd.3 mouse endothelioma cell line, loaded at 0.2 mg/mL. A specific band was detected for Endoglin/CD105 at approximately 121 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1320) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western blot shows lysates of bEnd.3 mouse endothelioma cell line and MS‑1 mouse pancreatic islet endothelial cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Endoglin/CD105 at approximately 90-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Endoglin/CD105 was detected in immersion fixed MS-1 mouse pancreatic islet endothelial cell line using Goat Anti-Mouse Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1320) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.