Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Draxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6148) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Draxin at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Draxin was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line using Sheep Anti-Human Draxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6148) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Draxin was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Sheep Anti-Human Draxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6148) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to diencephalon. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.