![Western Blot: p62/SQSTM1 Antibody [NBP1-42821] - HeLa cells were treated with (+) or without 50 uM (-) of Chloriquine (CQ) for 24 hours. Total cell lysates were prepared and separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane and blocked in 5% non-fat milk. The membrane was then probed with 2 ug/ml anti-p62/SQSMT1 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the upregulation of p62 (arrowhead) in response to chloroquine treatment and the blockage of autophagy.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_7/158_p62-SQSTM1-Antibody-Western-Blot-NBP1-42821-img0007.jpg)
Western Blot: p62/SQSTM1 Antibody [NBP1-42821] - HeLa cells were treated with (+) or without 50 uM (-) of Chloriquine (CQ) for 24 hours. Total cell lysates were prepared and separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane and blocked in 5% non-fat milk. The membrane was then probed with 2 ug/ml anti-p62/SQSMT1 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the upregulation of p62 (arrowhead) in response to chloroquine treatment and the blockage of autophagy.
![Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody [NBP1-42821] - Confocal immunofluorescent analysis of HeLa cells using p62/SQSTM1 antibody (NBP1-42821, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_7/159_p62-SQSTM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-42821-img0006.jpg)
Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody [NBP1-42821] - Confocal immunofluorescent analysis of HeLa cells using p62/SQSTM1 antibody (NBP1-42821, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
![Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody [NBP1-42821] - SQSTM1 staining in of Hela cells detected with a Dylight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_7/160_p62-SQSTM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-42821-img0005.jpg)
Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody [NBP1-42821] - SQSTM1 staining in of Hela cells detected with a Dylight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).