SETD1A Antibody

• Supplier: Novus Biologicals
• Catalog: NB100-558
• Prices: $359.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB ChIP IHC IHC-P IP
• Species Reactivities: Human, Bovine, Cat, Horse, Guinea Pig, Rabbit
• Antigen: A synthetic peptide which maps to a region between residues 1200 and 1250 of human hSET1 (KIAA0339) using the numbering given in TrEMBL entry O15047 (GeneID 9739).
• Purity/Format: Immunogen affinity purified
• Description: Rabbit Polyclonal
• Gene: SETD1A
• Conjugate: Unconjugated

Product images

Western Blot: SETD1A Antibody [NB100-558] - Detection of Human hSET1 by Western Blot. Samples: Nuclear extract (50, 15, 5 ug) from 293T cells. Antibody: Affinity purified rabbit anti-hSET1 antibody NB100-558 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry: SETD1A Antibody [NB100-558] - Sample: FFPE section of human ovarian carcinoma.~ Antibody: Affinity purified rabbit anti-hSet1 ~Cat. No. Lot4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

Chromatin Immunoprecipitation: SETD1A Antibody [NB100-558] - ChIP-chip scatter plot of anti-hSET1 (NB100-558) enriched DNA binding sites versus input reference DNA. A. 10 mcg of NB100-558 was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-hSET1 ChIP, normal rabbit IgG showed little enrichment.

Western Blot: SETD1A Antibody [NB100-558] - Nuclear extract (50 ug) from HeLa and 293T cells. Antibody: Affinity purified rabbit anti-hSET1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunohistochemistry-Paraffin: SETD1A Antibody [NB100-558] - FFPE section of human breast adenocarcinoma. Antibody: Affinity purified rabbit anti-hSET1 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.

Immunohistochemistry-Paraffin: SETD1A Antibody [NB100-558] - FFPE section of mouse squamous cell carcinoma. Antibody: Affinity purified rabbit anti-hSET1 used at a dilution of 1:250. Detection: DAB staining using immunohistochemistry Accessory Kit.

Immunohistochemistry-Paraffin: SETD1A Antibody [NB100-558] - FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-hSET1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunoprecipitation: SETD1A Antibody [NB100-558] - Detection of Human hSET1 on HeLa whole cell lysate using NB100-558. Affinity purified anti-hSET1 antibodies NB100-557, NB100-558, NB100-559, and another hSET1 antibody.