Western blot shows lysates of human motor cortex. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3154) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Sortilin at approximately 95-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
K562 human chronic myelogenous leukemia cell line was stained with Goat Anti-Human Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3154, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.
Human peripheral blood monocytes were stained with Goat Anti-Human Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3154, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.
Sortilin was detected in immersion fixed paraffin-embedded sections of human brain using Goat Anti-Human Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3154) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for Sortilin at approximately 82 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3154). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.