Western Blot: EPLIN Antibody [NB100-2305] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from MCF7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EPLIN antibody (lot was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: EPLIN Antibody [NB100-2305] - Detection of Human EPLIN by Western Blot. Samples: A) Whole cell lysate (50 mcg) from DU145 and SKBR3 cells. B) EPLIN that was immunoprecipitated from 500 mcg of whole cell lysate from DU145 cells. Antibodies: Affinity purified rabbit anti-EPLIN antibody NB100-2035 was used at 0.3 mcg/ml (A) or 1 mcg/ml (B) for WB. In B, EPLIN was immunoprecipitated using another rabbit anti-EPLIN antibody. Detection: Chemiluminescence with exposure times of 30 minutes (A) or 30 seconds (B).
Western Blot: EPLIN Antibody [NB100-2305] - Samples: Whole cell lysate (50 ug) from MCF7, HeLa, and 293T cells prepared using NETN lysis buffer. Antibody : Affinity purified rabbit anti-EPLIN antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.