![Western Blot: KMT2B Antibody [NB600-254] - analysis of MLL4 in Jurkat cell lysate (30ug/lane) using anti-MLL4 antibody. The primary antibody was used at a dilution of 1:2000 in PBS, incubated for 3 hours at room temperature. Note: MLL4 is degraded very fast and this could be why this antibody and other MLL4 antibodies may give smaller size band. Image from verified customer review.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_10/22049_KMT2B-Antibody-Western-Blot-NB600-254-img0004.jpg)
Western Blot: KMT2B Antibody [NB600-254] - analysis of MLL4 in Jurkat cell lysate (30ug/lane) using anti-MLL4 antibody. The primary antibody was used at a dilution of 1:2000 in PBS, incubated for 3 hours at room temperature. Note: MLL4 is degraded very fast and this could be why this antibody and other MLL4 antibodies may give smaller size band. Image from verified customer review.
![Chromatin Immunoprecipitation: KMT2B Antibody [NB600-254] - enriched DNA binding sites versus input reference DNA. A. 10 ug of this antibody was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-TRX2 ChIP, normal rabbit IgG showed little enrichment.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_10/22050_KMT2B-Antibody-Chromatin-Immunoprecipitation-NB600-254-img0002.jpg)
Chromatin Immunoprecipitation: KMT2B Antibody [NB600-254] - enriched DNA binding sites versus input reference DNA. A. 10 ug of this antibody was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-TRX2 ChIP, normal rabbit IgG showed little enrichment.
![Western Blot: KMT2B Antibody [NB600-254] - Detection of Human Trithorax 2 on HeLa whole cell lysate using NB600-254. Immunoprecipitated TRX2 was detected by WB using either NB600-254 or another rabbit anti-TRX2 antibody.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_10/22051_KMT2B-Antibody-Western-Blot-NB600-254-img0001.jpg)
Western Blot: KMT2B Antibody [NB600-254] - Detection of Human Trithorax 2 on HeLa whole cell lysate using NB600-254. Immunoprecipitated TRX2 was detected by WB using either NB600-254 or another rabbit anti-TRX2 antibody.
![Proximity Ligation Assay: KMT2B Antibody [NB600-254]](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_10/22052_KMT2B-Antibody-Proximity-Ligation-Assay-NB600-254-img0003.jpg)
Proximity Ligation Assay: KMT2B Antibody [NB600-254]
![Proximity Ligation Assay: KMT2B Antibody [NB600-254] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MEN1 (NB100-287) and rabbit anti-human TRX2 (NB600-254). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_10/22053_KMT2B-Antibody-Proximity-Ligation-Assay-NB600-254-img0007.jpg)
Proximity Ligation Assay: KMT2B Antibody [NB600-254] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MEN1 (NB100-287) and rabbit anti-human TRX2 (NB600-254). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.