![Western Blot: ROBO1 Antibody [NB600-1254] - Lane 1: HeLa WCL. Load: 30 ug per lane. Primary antibody: Robo-1 antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 181 kDa, 181 kDa for Robo-1. Other band(s): not identified.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_7/9241_ROBO1%2520Antibody-Western%2520Blot-NB600-1254-img0008.jpg)
Western Blot: ROBO1 Antibody [NB600-1254] - Lane 1: HeLa WCL. Load: 30 ug per lane. Primary antibody: Robo-1 antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 181 kDa, 181 kDa for Robo-1. Other band(s): not identified.
![Immunocytochemistry/Immunofluorescence: ROBO1 Antibody [NB600-1254] - Staining of Robo1 in undifferentiated, immortalized human podocytes by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 2% paraformaldehyde and 4% sucrose at room temperature for 10 minutes. The cells were then washed once with PBS, permeabilized with 0.3% Triton X-100 for 10 minutes and incubated with blocking solution (2% FCS, 2% BSA, 0.2% fish gelatin) for 30 minutes, before further incubation with primary Ab for 1 hour. An Alexa Fluor 488 goat anti-rabbit IgG secondary antibody was used at a dilution of 1/200. DAPI was used for nuclear counterstaining.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_7/9242_ROBO1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-1254-img0005.jpg)
Immunocytochemistry/Immunofluorescence: ROBO1 Antibody [NB600-1254] - Staining of Robo1 in undifferentiated, immortalized human podocytes by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 2% paraformaldehyde and 4% sucrose at room temperature for 10 minutes. The cells were then washed once with PBS, permeabilized with 0.3% Triton X-100 for 10 minutes and incubated with blocking solution (2% FCS, 2% BSA, 0.2% fish gelatin) for 30 minutes, before further incubation with primary Ab for 1 hour. An Alexa Fluor 488 goat anti-rabbit IgG secondary antibody was used at a dilution of 1/200. DAPI was used for nuclear counterstaining.
![Immunohistochemistry: ROBO1 Antibody [NB600-1254] - Used at a concentration of 5 ug/ml to detect ROBO1 in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows staining of human brain tissue. Tissue was formalin-fixed and paraffin embedded.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_7/9243_ROBO1-Antibody-Immunohistochemistry-NB600-1254-img0006.jpg)
Immunohistochemistry: ROBO1 Antibody [NB600-1254] - Used at a concentration of 5 ug/ml to detect ROBO1 in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows staining of human brain tissue. Tissue was formalin-fixed and paraffin embedded.
![Western Blot: ROBO1 Antibody [NB600-1254] - Detection of a band at ~181 kDa corresponding to ROBO-1 present in mouse brain lysate (arrowhead). Approximately 35 ug of lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye (TM) 800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_7/9244_ROBO1-Antibody-Western-Blot-NB600-1254-img0001.jpg)
Western Blot: ROBO1 Antibody [NB600-1254] - Detection of a band at ~181 kDa corresponding to ROBO-1 present in mouse brain lysate (arrowhead). Approximately 35 ug of lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye (TM) 800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
![Western Blot: ROBO1 Antibody [NB600-1254] - ylated human mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. ~20ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_7/9245_ROBO1-Antibody-Western-Blot-NB600-1254-img0003.jpg)
Western Blot: ROBO1 Antibody [NB600-1254] - ylated human mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. ~20ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.
![Immunohistochemistry-Frozen: ROBO1 Antibody [NB600-1254] - 1/50 staining mouse luug tissue sections (adult, frozen 100um wholemount sections) by IHC-Fr. The tissue was paraformaldehyde fixed and permeabilized with triton x-100 before incubation with the antibody for 16 hours at 4C.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_7/9246_ROBO1-Antibody-Immunohistochemistry-Frozen-NB600-1254-img0004.jpg)
Immunohistochemistry-Frozen: ROBO1 Antibody [NB600-1254] - 1/50 staining mouse luug tissue sections (adult, frozen 100um wholemount sections) by IHC-Fr. The tissue was paraformaldehyde fixed and permeabilized with triton x-100 before incubation with the antibody for 16 hours at 4C.