Western blot shows lysates of mouse embryo (15 d.p.c.) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat Semaphorin 4C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6120) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Semaphorin 4C at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of mouse embryo tissue (15 d.p.c.), loaded at 0.2 mg/mL. A specific band was detected for Semaphorin 4C at approximately 117 kDa (as indicated) using 50 µg/mL of Sheep Anti-Mouse/Rat Semaphorin 4C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6120) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Semaphorin 4C was detected in perfusion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Mouse/Rat Semaphorin 4C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6120) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes of the developing cerebellum. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Semaphorin 4C was detected in immersion fixed rat cortical stem cells using Sheep Anti-Mouse/Rat Semaphorin 4C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6120) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.