Musashi-2 Antibody (EP1305Y)

• Supplier: Novus Biologicals
• Catalog: NBP1-42029
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1305Y
• Applications: WB FC ICC/IF IHC IHC-P
• Species Reactivities: Human, Mouse, Rat
• Antigen: A synthetic peptide corresponding to N-terminus of human Musashi2 was used as an immunogen.
• Description: Rabbit Monoclonal
• Gene: MSI2

Product images

Western Blot: Musashi-2 Antibody (EP1305Y) [NBP1-42029] - (A) Rat brain (B) Human brain © SW480 and (D) T47D cell lysates.

Immunocytochemistry/Immunofluorescence: Musashi-2 Antibody (EP1305Y) [NBP1-42029] - Stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody , 1ug/ml) overnight at +4C. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM

Immunohistochemistry: Musashi-2 Antibody (EP1305Y) [NBP1-42029] - Analysis of paraffin-embedded human placenta tissue.

Flow Cytometry: Musashi-2 Antibody (EP1305Y) [NBP1-42029] - Overlay histogram showing HeLa cells stained with NBP1-42029 (red line).