MSI2

Gene Symbol MSI2
Entrez Gene 124540
Alt Symbol MSI2H
Species Human
Gene Type protein-coding
Description musashi RNA-binding protein 2
Other Description RNA-binding protein Musashi homolog 2|musashi homolog 2|musashi-2
Swissprots Q7Z6M7 Q96DH6 Q8N9T4
Accessions CBF67633 CBU92507 EAW94506 EAW94507 EAW94508 EAW94509 EAW94510 EAW94511 EAW94512 Q96DH6 AI798817 AK093888 BAC04244 AK295060 BAG58109 AK297295 BAG59763 BC001526 AAH01526 BC017560 AAH17560 BC035768 BE836557 BX647282 BX647939 DQ892317 ABM83243 DQ895519 ABM86445 XM_005257014 XP_005257071 XM_005257015 XP_005257072 XM_005257018 XP_005257075 XM_006721681 XP_006721744 XM_011524284 XP_011522586 XM_011524285 XP_011522587 XM_011524286 XP_011522588 XM_011524287 XP_011522589 NM_138962 NP_620412 NM_170721 NP_733839
Function RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity). {ECO:0000250}.
Subcellular Location Cytoplasm. Note=Associated with polysomes. {ECO:0000250}.
Tissue Specificity Ubiquitous; detected at low levels. {ECO:0000269|PubMed:12649177}.
Top Pathways mRNA surveillance pathway

Msi2 (H-40) - sc-367844 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Msi2 (H-40): sc-367844. Western blot analysis of Msi2 expression in mouse brain tissue extract.

Msi2 (T-16) - sc-83160 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Msi2 (T-16): sc-83160. Western blot analysis of Msi2 expression in IMR-32 (A) and HL-60 (B) whole cell lysates.

Anti-MSI2 antibody - ab116272 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MSI2 antibody (ab116272) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Brain (Mouse) Tissue LysateLane 3 : Brain (Rat) Tissue LysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-MSI2 antibody - ab105889 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MSI2 antibody (ab105889) at 1 µg/mlLane 1 : Brain (Mouse) Tissue LysateLane 2 : Spinal Cord (Mouse) Tissue LysateLane 3 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-MSI2 antibody - ab57341 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab57341 MSI2 antibody at 1ug/ml.

Anti-MSI2 antibody - ab114107 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Detection of Human MSI2 by Western blot of Immunoprecipitate. MSI2 was immunoprecipitated from HeLa whole cell lysate, using ab114107 at 6 µg/mg lysate (1 mg for IP, 20% of IP loaded). ab114107 was used for the subsequent WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-MSI2 antibody [2C11] - ab113678 from Abcam

Host Mouse
Clonality Monoclonal
Clone 2C11
All lanes : Anti-MSI2 antibody [2C11] (ab113678) at 1/500 dilutionLane 1 : NTERA-2 cell lysateLane 2 : SW620 cell lysateLane 3 : T47D cell lysate

Anti-MSI2 antibody [2F10] - ab156770 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2F10
All lanes : Anti-MSI2 antibody [2F10] (ab156770) at 1/4000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY MSI2 cDNALysates/proteins at 5 µg per lane.

Anti-MSI2 antibody [EP1305Y] - ab76148 from Abcam

Prices $391.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1305Y
All lanes : Anti-MSI2 antibody [EP1305Y] (ab76148) at 1/2000 dilutionLane 1 : Rat brain cell lysates Lane 2 : Human brain cell lysates Lane 3 : SW480 cell lysates Lane 4 : T47D cell lysates Lysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-MSI2 antibody [EP1305Y] (Alexa Fluor® 488) - ab199067 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1305Y
ab199067 staining MSI2 in PC12 cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199067 at 1/50 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-MSI2 antibody [EP1305Y] (Alexa Fluor® 647) - ab199251 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1305Y
ab199251 staining MSI2 in PC12 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199251 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-MSI2 antibody - N-terminal - ab195990 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-MSI2 antibody - N-terminal (ab195990) at 2 µg/ml + Kelly lysate (in RIPA buffer) at 35 µgdeveloped using the ECL technique

Anti-MSI2 Antibody - LS-C334451 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of mouse brain, using MSI2 antibody.

Anti-MSI2 Antibody (clone 2C11) - LS-C169328 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2C11
IHC of paraffin-embedded ovarian cancer (left) and cerebellum tissues (right) using MSI2 mouse monoclonal antibody with DAB staining.

Anti-MSI2 Antibody (clone 1F2) - LS-C139535 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1F2

Anti-MSI2 Antibody (N-Terminus) - LS-C101889 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
MSI2 antibody LS-C101889 Western blot of HeLa lysate.

Anti-MSI2 Antibody (aa37-86) - LS-C31558 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
MSI2 antibody LS-C31558 was used in IHC to stain formalin-fixed, paraffin-embedded human heart.

Anti-MSI2 Antibody (C-Terminus) - LS-C110611 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
MSI2 antibody LS-C110611 Western blot of Fetal Small Intestine lysate.

Anti-MSI2 Antibody (aa200-250) - LS-C289910 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-MSI2 Antibody (aa250-300) - LS-C289912 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-MSI2 Antibody (N-Terminus, clone EP1305Y) - LS-C105360 from LifeSpan Bioscience

Prices $490.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1305Y
IHC of paraffin-embedded human placenta using anti-Musashi2 antibody (LS-C105360).

Anti-MSI2 Antibody (N-Terminus) IHC-plus™ - LS-B7105 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-MSI2 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7105 concentration 5 ug/ml.

Anti-MSI2 Antibody (clone 2F10) - LS-C174732 from LifeSpan Bioscience

Prices $350.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2F10
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MSI2 mouse monoclonal antibody.

Musashi-2 Antibody (EP1305Y) - NBP1-42029 from Novus Biologicals

Host Rabbit
Clonality Monoclonal
Clone EP1305Y
Western Blot: Musashi-2 Antibody (EP1305Y) [NBP1-42029] - (A) Rat brain (B) Human brain © SW480 and (D) T47D cell lysates.

Musashi-2 Antibody [Unconjugated] - AF3255 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Goat
Clonality Polyclonal
Musashi‑2 was detected in immersion fixed undifferentiated rat cortical stem cells using Goat Anti-Human Musashi‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3255) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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