Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 8828
• Prices: $287.00
• Sizes: 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D27F4
• Applications: WB ICC/IF FC
• Species Reactivities: Human, Mouse, Rat, Monkey
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser465/467 of human Smad2 protein.
• Description: Rabbit Monoclonal
• Gene: SMAD2

Product images

Western blot analysis of extracts from HaCaT cells, untreated (-) or treated with hTGF-β3 #8425 (+) in the absence or presence of the TGFR inhibitor SB 431542, using Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb (upper) or Smad2/3 (D7G7) XP ® Rabbit mAb #8685 (lower).

Confocal immunofluorescent analysis of HaCaT cells, serum-starved (left), treated with hTGF-β3 #8425 (100 ng/ml, 30 min; center), or treated with hTGF-β3 and SB 431542 (10 μg/mL, 1 hr; right), using Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb (green) and COX IV (3E11) Rabbit mAb (Alexa Fluor ® 555 Conjugate) #8693 (red).

Flow cytometric analysis of HT-1080 cells, untreated (blue), treated with hTGF-β3 #8425 (green), or treated with hTGF-β3 and SB 431542 (red), using Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb.