Western Blot: KPNA3 Antibody [NB100-81650] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-81650 used for WB at 0.4 mcg/ml (A and B) and used for IP at 3 mcg/mg lysate.
Immunohistochemistry-Paraffin: KPNA3 Antibody [NB100-81650] - Human prostate carcinoma. Antibody used at a dilution of 1:200.
Immunohistochemistry-Paraffin: KPNA3 Antibody [NB100-81650] - FFPE section of human ovarian carcinoma. Affinity purified rabbit anti-KPNA3 used at a dilution of 1:250.
Proximity Ligation Assay: KPNA3 Antibody [NB100-81650] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RCC1 (NB110-40590) and rabbit anti-human KPNA3 (NB100-81650). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.
Proximity Ligation Assay: KPNA3 Antibody [NB100-81650] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human HdmX (NBP2-22228) and rabbit anti-human KPNA3 (NB100-81650). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.