Smad2 (D43B4) XP ® Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 5339
• Prices: $109.00, $277.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D43B4
• Applications: WB IP ICC/IF FC ChIP
• Species Reactivities: Human, Mouse, Rat, Monkey
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse Smad2 protein.
• Description: Rabbit Monoclonal
• Gene: SMAD2

Product images

Western blot analysis of extracts from various cell lines using Smad2 (D43B4) XP ® Rabbit mAb.

Confocal immunofluorescent analysis of NIH/3T3 cells, serum-starved (left) or treated with hTGF-β3 #8425 (right), using Smad2 (D43B4) XP ® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Flow cytometric analysis of HeLa cells using Smad2 (D43B4) XP ® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HaCaT cells treated with Human TGF-β3 #8425 (7 ng/ml) for 1 h and either 10 μl of Smad2 (D43B4) XP ® Rabbit mAb #5339 or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human CDKN1A Intron 1 Primers #4669, SimpleChIP ® Human ID1 Promoter Primers #5139, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.