Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 9520
• Prices: $109.00, $297.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: C25A9
• Applications: WB IP ChIP
• Species Reactivities: Human, Mouse, Rat, Monkey, Xenopus, Zebrafish, Bovine
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser423/425 of Smad3.
• Description: Rabbit Monoclonal
• Gene: SMAD3

Product images

Western blot analysis of extracts from HT-1080, C2C12, or KNRK cells, untreated (-) or treated with TGF-β (10 ng/ml, 30 min; +), using Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb (upper) or total Smad3 (C67H9) Rabbit mAb #9523 (lower).

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HaCaT cells treated with Human TGF-β3 #3706 (7 ng/ml) for 1 h and either 5 μl of Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human CDKN1A Intron 1 Primers #4669, SimpleChIP ® Human ID1 Promoter Primers #5139, human c-Myc intron 1 primers, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.