Smad3 (C67H9) Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 9523
• Prices: $99.00, $246.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: C67H9
• Applications: WB IP ICC/IF FC ChIP
• Species Reactivities: Human, Mouse, Rat, Monkey, Xenopus, Zebrafish, Bovine
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues at the amino terminus of Smad3.
• Description: Rabbit Monoclonal
• Gene: SMAD3

Product images

Western blot analysis of extracts from HT1080 (human), C2C12 (mouse) and B35 (rat) using Smad3 (C67H9) Rabbit mAb.

Western blot analysis of extracts from HT1080 cells, treated with TGF-β1, TGFR inhibitor SB-431542 or BMP-2, using Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb #9520 (upper) or total Smad3 (C67H9) Rabbit mAb #9523 (lower).

Confocal immunofluorescent analysis of HT1080 cells, untreated (left) or TGFβ-treated (right), using Smad3 (C67H9) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor ® 555 phalloidin (red).

Flow cytometric analysis of HT-1080 cells using Smad3 (C67H9) Rabbit mAb #9523 (blue) compared to a nonspecific negative control antibody (red).

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HaCaT cells treated with Human TGF-β3 #3706 (7 ng/ml) for 1 h and either 10 μl of Smad3 (C67H9) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human CDKN1A Intron 1 Primers #4669, SimpleChIP ® Human ID1 Promoter Primers #5139, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.