Western Blot: CPSF1 Antibody [NB100-79822] - Detection of Human and Mouse CPSF160 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CPSF160 antibody NB100-79822 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. CPSF160 was also immunoprecipitated by rabbit another anti-CPSF160 antibody, which recognizes an upstream epitope.
Immunocytochemistry/Immunofluorescence: CPSF1 Antibody [NB100-79822] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CPSF160 used at a dilution of 1:100. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1:100.
Immunohistochemistry-Paraffin: CPSF1 Antibody [NB100-79822] - Human ovarian carcinoma (left) and mouse renal cell carcinoma (right). Antibody used at a dilution of 1:200 (1ug/ml).
Proximity Ligation Assay: CPSF1 Antibody [NB100-79822] - Secondary-conjugate Duolink In Situ PLA in MCF-7 cells. goat anti-human MCM2 (NB100-244) and rabbit anti-human CPSF160 (NB100-79822). Image merged from DAPI (2ms), FITC/Green (25ms) and TRITC/Orange (250ms) exposures, 40X magnification.
Proximity Ligation Assay: CPSF1 Antibody [NB100-79822] - Secondary-conjugate Duolink In Situ PLA in MCF-7 cells. goat anti-human POLR2A (NBP2-14859) and rabbit anti-human CPSF160 (NB100-79822). Image merged from DAPI (2ms), FITC/Green (25ms) and TRITC/Orange (250ms) exposures, 40X magnification.