Western Blot: PHD4/HIF Prolyl Hydroxylase 4 Antibody [NB100-295] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHD4 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: PHD4/HIF Prolyl Hydroxylase 4 Antibody [NB100-295] - Analysis of human PHD4, using NB100-295. Samples: whole cell lysate from HeLa cells, normoxic and hypoxic, respectively.
Western Blot: PHD4/HIF Prolyl Hydroxylase 4 Antibody [NB100-295] - Whole cell lysate (25 mcg/lane) from human glioblastoma tumor cell lines or in vitro translated PHD4. Antibody: Affinity purified rabbit anti-PHD4 (Cat. No. NB100-295) used at 2 mcg/ml. Detection: Chemiluminescence