Smad5 (D4G2) Rabbit mAb
| Name | Smad5 (D4G2) Rabbit mAb |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 12534 |
| Prices | $246.00 |
| Sizes | 100 µl (10 western blots) |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | D4G2 |
| Applications | WB IP ChIP |
| Species Reactivities | Human, Mouse, Rat, Monkey |
| Antigen | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro249 of human Smad5 protein. |
| Description | Rabbit Monoclonal |
| Gene | SMAD5 |
| Supplier Page | Shop |
Product images
Western blot analysis of extracts from various cell lines using Smad5 (D4G2) Rabbit mAb.
Immunoprecipitation of Smad5 from HT-1080 cell extracts using Normal Rabbit IgG #2729 (lane 2) or Smad5 (D4G2) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Smad5 (D4G2) Rabbit mAb.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 MCF7 cells treated with Human BMP2 #4697 (50 ng/ml, 1 hr) and either 5 μl of Smad5 (D4G2) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human ID1 Promoter Primers #5139, human Smad6 promoter primers, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.