Western blot shows lysates of DU145 human prostate carcinoma cell line, HT‑29 human colon adenocarcinoma cell line, and HT‑29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human PTPN14 Monoclonal Antibody (Catalog # MAB4458) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PTPN14 at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PTPN14 was detected in immersion fixed paraffin-embedded sections of human placenta using Human PTPN14 Monoclonal Antibody (Catalog # MAB4458) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.