Western blot shows lysates of human intestine tissue and mouse intestine tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Meprin beta Subunit/MEP1B Monoclonal Antibody (Catalog # MAB28951) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Meprin beta Subunit/MEP1B at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Meprin beta Subunit/MEP1B was detected in immersion fixed paraffin-embedded sections of human intestine using Human/Mouse Meprin beta Subunit/MEP1B Monoclonal Antibody (Catalog # MAB28951) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hemotoxylin (blue). Specific staining was localized to the Brush border of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. This application has not been tested in mouse samples