Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and EL‑4 mouse lymphoblast cell line. Gels were loaded with 20 µg of HepG2 and 30 µg of EL-4 cytoplasmic (Cyto) extracts and 10 µg of HepG2 and 15 µg of EL-4 nuclear (Nuc) extracts. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SART1 Monoclonal Antibody (Catalog # MAB8034) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SART1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
SART1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human SART1 Monoclonal Antibody (Catalog # MAB8034) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.