Western blot shows lysates of human brain (cerebellum) tissue, mouse brain (cortex) tissue, and rat brain (cerebellum) tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/RatComplexin-1/2 Monoclonal Antibody (Catalog # MAB8069) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Complexin-1/2 at approximately 17-19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.5 mg/mL. A specific band was detected for Complexin-1/2 at approximately 23 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Mouse/Rat Complexin-1/2 Monoclonal Antibody (Catalog # MAB8069). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Complexin-1/2 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human/Mouse/Rat Complexin-1/2 Monoclonal Antibody (Catalog # MAB8069) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.