Western blot shows lysates of Jurkat human acute T cell leukemia cell line, SK‑OV‑3 human ovarian adenocarcinoma cell line, ZR‑75 human breast cancer cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Rat GOLGB1/Giantin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8159) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GOLGB1/Giantin at approximately 370 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
GOLGB1/Giantin was detected in 2% paraformaldehyde immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Rat GOLGB1/Giantin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8159) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to Golgi apparatus vesicles. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.