![Western blot shows lysates of HEK293 human embryonic kidney cell line, HT1080 human fibrosarcoma cell line, and HL‑60 human acute promyelocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human TBX6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4744) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TBX6 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_10/22093_TBX6_AF4744_Western_Blot_5538.jpg)
Western blot shows lysates of HEK293 human embryonic kidney cell line, HT1080 human fibrosarcoma cell line, and HL‑60 human acute promyelocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human TBX6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4744) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TBX6 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
![TBX6 was detected in immersion fixed frozen sections of embryonic mouse mesoderm (E9.5) using 10 µg/mL Human TBX6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4744) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_10/22094_TBX6_AF4744_Immunohistochemistry_6843.jpg)
TBX6 was detected in immersion fixed frozen sections of embryonic mouse mesoderm (E9.5) using 10 µg/mL Human TBX6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4744) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.