Necdin Antibody (1B3)

• Supplier: Novus Biologicals
• Catalog: H00004692-M02
• Prices: $359.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a Kappa
• Clone: 1B3
• Applications: WB ELISA ICC/IF IHC-P Gene Silencing
• Species Reactivities: Human
• Antigen: NDN (NP_002478 222 a.a. - 321 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. WKKHSTFGDVRKLITEEFVQMNYLKYQRVPYVEPPEYEFFWGSRASREITKMQIMEFLARVFKKDPQAWPSRYREALEEARALREANPTAHYPRSSVSED
• Purity/Format: IgG purified
• Description: Mouse Monoclonal
• Gene: NDN
• Conjugate: Unconjugated

Product images

Western Blot: Necdin Antibody (1B3) [H00004692-M02] - NDN monoclonal antibody (M02), clone 1B3 Analysis of NDN expression in HL-60.

Immunocytochemistry/Immunofluorescence: Necdin Antibody (1B3) [H00004692-M02] - Analysis of monoclonal antibody to NDN on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: Necdin Antibody (1B3) [H00004692-M02] - Analysis of monoclonal antibody to NDN on formalin-fixed paraffin-embedded human colon. Antibody concentration 1.5 ug/ml.

Western Blot: Necdin Antibody (1B3) [H00004692-M02] - Analysis of NDN over-expressed 293 cell line, cotransfected with NDN Validated Chimera RNAi ( Cat # H00004692-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NDN monoclonal antibody (M02), clone 1B3 (Cat # H00004692-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: Necdin Antibody (1B3) [H00004692-M02] - Analysis of NDN expression in transfected 293T cell line by NDN monoclonal antibody (M02), clone 1B3.Lane 1: NDN transfected lysate(36.1 KDa).Lane 2: Non-transfected lysate.

ELISA: Necdin Antibody (1B3) [H00004692-M02] - Detection limit for recombinant GST tagged NDN is approximately 0.03ng/ml as a capture antibody.