Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HCF1 antibody NB100-68209 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68210, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Proximity Ligation Assay: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MEN1 (NB100-287) and rabbit anti-human HCF1 (NB100-68209). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.
Proximity Ligation Assay: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SDS3 (NB100-257) and rabbit anti-human HCF1 (NB100-68209). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.