Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, PC‑3 human prostate cancer cell line, and Caki‑2 human clear cell carcinoma epithelial cell line. PVDF Membrane was probed with 0.5 µg/mL of Human Palladin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5950) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Palladin at approximately 95, 115, and 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Palladin was detected in immersion fixed paraffin-embedded sections of human prostate using Human Palladin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5950) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.