Western blot shows lysates of BG0IV human embryonic stem cells and NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human LIN‑41 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5104) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LIN‑41 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.2 mg/mL. A specific band was detected for LIN‑41 at approximately 97 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human LIN‑41 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5104) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
LIN‑41 was detected in immersion fixed BG01V human embryonic stem cells using Human LIN‑41 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5104) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.