Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 20 ng/mL Recombinant Human IFN‑ gamma (Catalog # 285‑IF) and 20 ng/mL Recombinant Human TNF‑ alpha (Catalog # 210‑TA) for 6 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL‑36 gamma /IL‑1F9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2320) followed by HRP‑conjugated Anti‑Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑36 gamma /IL‑1F9 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.