Western blot shows lysates of human lung tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human Laminin alpha 3/Laminin‑5 Monoclonal Antibody (Catalog # MAB21441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Laminin alpha 3/Laminin‑5 at approximately 190 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Laminin alpha 3/Laminin‑5 was detected in immersion fixed NHEK human normal epidermal keratinocytes treated with 10ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B) for 24 hours using Mouse Anti-Human Laminin alpha 3/Laminin‑5 Monoclonal Antibody (Catalog # MAB21441) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the perinuclear space. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.