Western blot shows lysates of HT‑29 human colon adenocarcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human Protocadherin‑8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5434) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Protocadherin‑8 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Protocadherin‑8 was detected in immersion fixed MCF‑7 human breast cancer cell line using Human Protocadherin‑8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5434) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.