Western Blot: BLAP75 Antibody [NB100-1720] - Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-BLAP75 antibody NB100-1720 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).
Immunohistochemistry: BLAP75 Antibody [NB100-1720] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BLAP75 used at a dilution of 1:200 (1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunohistochemistry-Paraffin: BLAP75 Antibody [NB100-1720] - FFPE section of human skin basal cell carcinoma. NB100-1720 used at a dilution of 1:250. DAB staining using anti-Rabbit IHC antibody at a dilution of 1:100.
Proximity Ligation Assay: BLAP75 Antibody [NB100-1720] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RPA2 (NBP2-22232) and rabbit anti-human BLAP75 (NB100-1720). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.
Proximity Ligation Assay: BLAP75 Antibody [NB100-1720] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anti-human BLAP75 (NB100-1720).Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.