Western Blot: NCOA2 Antibody - hole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-NCOA2 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: NCOA2 Antibody [NBP2-22162] - FFPE section of prostate adenocarcinoma. Antibody used at a dilution of 1:100. Detection: DAB
Immunohistochemistry-Paraffin: NCOA2 Antibody [NBP2-22162] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-NCOA2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: NCOA2 Antibody [NBP2-22162] - FFPE section of human breast adenocarcinoma. Antibody used at a dilution of 1:250. Detection: DAB
Immunoprecipitation: NCOA2 Antibody - Nuclear extract (A, 10 mg) or cytosolic (S100) extract (B, 10 mg) from HeLa cells. Antibody: Affinity purified rabbit NCOA2 used at 20 ug/10 mg lysate. Detection: Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is NCOA2.