![Western Blot: NEDD9/CASL/HEF1 Antibody (2G9) [NB100-1699] - Detection of endogenous HEF1 present in various cell lines. Panel A shows detection using a 15 min exposure. Panel B is the same blot exposed for 2 min. The doublet represents p105 and p115 staining. The lower MW band represents p55. 3Y1 cells are derived from rat fibroblast cells. Mouse 3T3 cells are also reactive (not shown). To date no staining has been noted on CHO cells.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_8/298_NEDD9-CASL-HEF1-Antibody-(2G9)-Western-Blot-NB100-1699-img0010.jpg)
Western Blot: NEDD9/CASL/HEF1 Antibody (2G9) [NB100-1699] - Detection of endogenous HEF1 present in various cell lines. Panel A shows detection using a 15 min exposure. Panel B is the same blot exposed for 2 min. The doublet represents p105 and p115 staining. The lower MW band represents p55. 3Y1 cells are derived from rat fibroblast cells. Mouse 3T3 cells are also reactive (not shown). To date no staining has been noted on CHO cells.
![Immunocytochemistry/Immunofluorescence: NEDD9/CASL/HEF1 Antibody (2G9) [NB100-1699] - Detection of HEF1 localized at focal adhesion sites. The antibody was used at a 1:500 dilution with a 3-sec exposure time.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_8/299_NEDD9-CASL-HEF1-Antibody-(2G9)-Immunocytochemistry-Immunofluorescence-NB100-1699-img0009.jpg)
Immunocytochemistry/Immunofluorescence: NEDD9/CASL/HEF1 Antibody (2G9) [NB100-1699] - Detection of HEF1 localized at focal adhesion sites. The antibody was used at a 1:500 dilution with a 3-sec exposure time.
![Western Blot: NEDD9/CASL/HEF1 Antibody (2G9) [NB100-1699] - Detection of a 115 kDa band corresponding to HEF1 in MCF7 lysate (arrowhead). Approximately 35 ig of lysate was loaded for SDS-PAGE followed by transfer onto nitrocellulose and reaction with a 1:1,000 dilution of anti-HEF1 antibody. Detection occurred using a 1:5,000 dilution of IRDye800 conjugated Sh-a-Mouse IgG [H&L] (610-632-002) for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers (indicated at left). IRDye800 fluorescence image was captured using the OdysseyInfrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_8/300_NEDD9-CASL-HEF1-Antibody-(2G9)-Western-Blot-NB100-1699-img0007.jpg)
Western Blot: NEDD9/CASL/HEF1 Antibody (2G9) [NB100-1699] - Detection of a 115 kDa band corresponding to HEF1 in MCF7 lysate (arrowhead). Approximately 35 ig of lysate was loaded for SDS-PAGE followed by transfer onto nitrocellulose and reaction with a 1:1,000 dilution of anti-HEF1 antibody. Detection occurred using a 1:5,000 dilution of IRDye800 conjugated Sh-a-Mouse IgG [H&L] (610-632-002) for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers (indicated at left). IRDye800 fluorescence image was captured using the OdysseyInfrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.