NEDD9

Gene Symbol NEDD9
Entrez Gene 4739
Alt Symbol CAS-L, CAS2, CASL, CASS2, HEF1
Species Human
Gene Type protein-coding
Description neural precursor cell expressed, developmentally down-regulated 9
Other Description Cas scaffolding protein family member 2|Crk-associated substrate related protein Cas-L|Enhancer of filamentation 1 p55|cas-like docking|enhancer of filamentation 1|neural precursor cell expressed developmentally down-regulated protein 9|p130Cas-related protein|renal carcinoma antigen NY-REN-12
Swissprots Q5XKI0 A8MSJ9 A8K9G7 Q14511 G5E9Y9 Q5T9R4
Accessions EAW55299 EAW55300 EAW55301 EAW55302 EAW55303 Q14511 AA814628 AJ420493 AK292682 BAF85371 AW963532 BC020686 AAH20686 BC040207 AAH40207 BC050740 BP262776 BX648041 DA660812 DA676780 DA778416 DQ891725 ABM82651 DQ894902 ABM85828 EU832525 ACE87697 EU832607 ACE87009 L43821 AAA98770 U64317 AAB53696 NM_001142393 NP_001135865 NM_001271033 NP_001257962 NM_006403 NP_006394 NM_182966 NP_892011 NR_073131
Function Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. May function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form.
Subcellular Location Cytoplasm, cell cortex. Nucleus. Golgi apparatus. Cell projection, lamellipodium. Cytoplasm. Cell junction, focal adhesion. Note=Localizes to both the cell nucleus and the cell periphery and is differently localized in fibroblasts and epithelial cells. In fibroblasts is predominantly nuclear and in some cells is present in the Golgi apparatus. In epithelial cells localized predominantly in the cell periphery with particular concentration in lamellipodia but is also found in the nucleus. Isoforms p105 and p115 are predominantly cytoplasmic and associate with focal adhesions while p55 associates with mitotic spindle.
Tissue Specificity Widely expressed. Higher levels detected in kidney, lung, and placenta. Also detected in T-cells, B-cells and diverse cell lines. The protein has been detected in lymphocytes, in diverse cell lines, and in lung tissues.

HEF1/NEDD9 (2G9) Mouse mAb - 4044 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 2G9
Western blot analysis of extracts from A549 and C2C12 cell lines using HEF1/NEDD9 (2G9) Mouse mAb.

Cas-L (N-17) - sc-6848 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Cas-L (N-17): sc-6848. Western blot analysis of Cas-L expression in HeLa whole cell lysate.

Cas-L (2G9) - sc-33659 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cas-L (2G9): sc-33659. Western blot analysis of Cas-L expression in CTLL-2 whole cell lysate.

Cas-L (2B11) - sc-33658 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cas-L (2B11): sc-33658. Western blot analysis of Cas-L expression in CTLL-2 whole cell lysate.

Cas-L (H-70) - sc-25503 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Cas-L (H-70): sc-25503. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.

Cas-L (14A11) - sc-33657 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cas-L (14A11): sc-33657. Western blot analysis of Cas expression in MCF7 (A) and HuT 78 (B) whole cell lysates. Note presence of C-terminal cleavage product.

Cas-L (L-15) - sc-31866 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Cas-L (L-15): sc-31866. Western blot analysis of Cas-L expression in non-transfected: sc-117752 (A) and human Cas-L transfected: sc-159485 (B) 293T whole cell lysates.

Anti-HEF1 (phospho S369) antibody - ab110854 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HEF1 antibody - ab37161 from Abcam

Prices $376.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HEF1 antibody (ab37161) at 1/250 dilutionLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human HEF1 peptide (ab39117) at 1 µg/mlLane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899) with Human HEF1 peptide (ab39117) at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-HEF1 antibody - ab88584 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-HEF1 antibody (ab88584) at 1 µg/ml + 293 cell lysate at 50 µgSecondaryGoat anti-Mouse IgG (H+L) HRP at 1/5000 dilution

Anti-HEF1 antibody - ab106831 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-HEF1 antibody (ab106831) at 0.1 µg/ml + Human kidney lysate (in RIPA buffer) at 35 µgdeveloped using the ECL technique

Anti-HEF1 antibody - ab166663 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-HEF1 antibody (ab166663) at 1/1000 dilutionLane 1 : Mouse thymus whole cell extractLane 2 : HeLa whole cell extractLane 3 : Jurkat whole cell extract

Anti-HEF1 antibody - ab184627 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-HEF1 antibody (ab184627) at 1/1000 dilution + BCL-1 whole cell lysate at 30 µg

Anti-HEF1 antibody [14A11] - ab18059 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 14A11

Anti-HEF1 antibody [2G9] - ab18056 from Abcam

Prices $403.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2G9
ICC/IF image of ab18056 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18056, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

NEDD9/CASL/HEF1 Antibody (2G9) - NB100-1699 from Novus Biologicals

Prices $349.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2G9
Western Blot: NEDD9/CASL/HEF1 Antibody (2G9) [NB100-1699] - Detection of endogenous HEF1 present in various cell lines. Panel A shows detection using a 15 min exposure. Panel B is the same blot exposed for 2 min. The doublet represents p105 and p115 staining. The lower MW band represents p55. 3Y1 cells are derived from rat fibroblast cells. Mouse 3T3 cells are also reactive (not shown). To date no staining has been noted on CHO cells.

NEDD9/CASL/HEF1 Antibody (584825) - MAB5459 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 584825
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human NEDD9/CASL Monoclonal Antibody (Catalog # MAB5459) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for NEDD9/CASL at approximately 110 kDa & 113 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

NEDD9/CASL/HEF1 Antibody - AF5459 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Goat
Clonality Polyclonal
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human NEDD9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5459) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for NEDD9 at approximately 105 and 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

NEDD9/CASL/HEF1 Antibody - H00004739-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: NEDD9/CASL/HEF1 Antibody [H00004739-B01P] - Analysis of NEDD9 expression in 293.

NEDD9/CASL/HEF1 Antibody - H00004739-D01 from Novus Biologicals

Prices $399.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: HEF1 Antibody [H00004739-D01] - NEDD9 MaxPab rabbit polyclonal antibody. Western Blot analysis of NEDD9 expression in mouse brain.

NEDD9/CASL/HEF1 Antibody - H00004739-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: NEDD9/CASL/HEF1 Antibody [H00004739-D01P] - Analysis of NEDD9 expression in mouse brain.

NEDD9/CASL/HEF1 Antibody (1B4) - H00004739-M01 from Novus Biologicals

Prices $349.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1B4
Western Blot: NEDD9/CASL/HEF1 Antibody (1B4) [H00004739-M01] - Analysis of NEDD9 expression in transfected 293T cell line by NEDD9 monoclonal antibody (M01), clone 1B4.Lane 1: NEDD9 transfected lysate(92.9 KDa).Lane 2: Non-transfected lysate.

NEDD9/CASL/HEF1 Antibody - NBP2-37887 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

NEDD9/CASL/HEF1 Antibody - NB100-68151 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: NEDD9/CASL/HEF1 Antibody [NB100-68151] - Staining of Human Kidney lysate.

NEDD9/CASL/HEF1 Antibody (1B4) - H00004739-M01A from Novus Biologicals

Prices $349.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Clone 1B4
Search more