Western Blot: GLDC Antibody [NBP1-32907] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: GLDC Antibody [NBP1-32907] - Hep G2 cells were fixed in ice-cold MeOH for 5 min. Green: Glycine dehydrogenase protein stained by Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:400. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-32907] - Paraffin-embedded mouse brain. Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:500.
Western Blot: GLDC Antibody [NBP1-32907] - A. 30 ug HeLa whole cell lysate/extract. B. 30 ug HepG2 whole cell lysate/extract.
Immunocytochemistry/Immunofluorescence: GLDC Antibody [NBP1-32907] - Methanol-fixed HeLa, using Glycine dehydrogenase antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-32907] - Paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-32907] - Paraffin-embedded mouse liver. Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:500.
Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-32907] - Paraffin-embedded rat brain. Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:500
Simple Western: GLDC Antibody [NBP1-32907] - Simple Western lane view shows a specific band for GLDC in 0.2 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Immunoprecipitation: GLDC Antibody [NBP1-32907] - Immunoprecipitation of Glycine dehydrogenase protein from HepG2 whole cell extracts using 5 ug of Glycine dehydrogenase antibody [N3C2-2], Internal. Western blot analysis was performed using Glycine dehydrogenase antibody [N3C2-2], Internal. EasyBlot anti-Rabbit IgG(was used as a secondary reagent.